| Specific Usage: | For Microbiological |
|---|---|
| Usage: | Laboratory Reagents |
| Source: | Dry Powder |
| Application: | Industry, Scientific Research, Health, Environmental Protection |
| Property: | Biochemical Reagent |
| Specification: | 250g/bottle |
| Samples: |
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| Customization: |
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Plate Count Agar is used for the cultivation of bacteria and for the enumeration of microorganisms in water, sewage, faeces and other materials. The medium contains sugar.
Approximate Formula:
|
Ingredients |
gm/liter |
|
Pancreatic digest of casein |
5.0 |
|
Yeast extract |
2.5 |
|
Dextrose |
1.0 |
|
Agar |
15.0 |
|
Final pH7.0±0.2 at 25ºC |
|
|
*Adjusted and/or supplemented as required to meet performance criteria. |
|
Directions:
Suspend 23.5g of the medium in one liter of deionized or distilled water. Mix well and heat with frequent agitation. Boil for 1 minute. Dispense into tubes or flasks. Sterilize in the autoclave at 121ºC(15 lbs.) for 15 minutes.
Principle and Interpretation:
Plate Count Agar is used for the cultivation of bacteria and for the enumeration of organisms in water, sewage, faeces and other materials. The medium contains sugar. Plate Count Agar is equivalent to the medium recommended by the APHA, PHLS and ISO for the plate count of micro-organisms in food. The APHA method consists of the preparation of pour plates using diluted samples, and counting colonies after incubation.
Pancreatic digest of caseinprovides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B complex. APHA recommends the use of pour plate technique. The samples are diluted and appropriate dilutions are added in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count Agar is also suitable for enumerating bacterial count of sterile rooms.
Appearance:
Dehydrated medium is a free-flowing yellowish powder. The prepared medium is a kind of yellowish transparent gel.
Precautions:
This medium is for laboratory use only. Dried medium which is past shelf life, caking or color variation cannot be used.
Storage conditions and Shelf life:
Plate Count Agar must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.
Quality control:
Cultural characteristics observed after an incubation at 35 -37°C for 18 - 48 hours.
|
Microorganism |
Strains Number |
Inoculum (CFU) |
Growth |
Recovery |
Remarks |
|
Pseudomonas aeruginosa |
ATCC 9027 |
20-200 |
luxuriant |
≥70% |
Colorless transparent colonies |
|
Escherichia coli |
ATCC 25922 |
20-200 |
luxuriant |
≥70% |
Colorless transparent large colonies |
|
Bacillus subtilis |
ATCC 6633 |
20-200 |
luxuriant |
≥70% |
White irregular colonies |
|
Bacillus cereus |
ATCC 11778 |
20-200 |
luxuriant |
≥70% |
White irregular colonies |
|
Staphylococcus Aureus |
ATCC 6538 |
20-200 |
luxuriant |
≥70% |
Yellowish colonies |
Reference:
1. Buchbinder L., Baris Y., Aldd E., Reynolds E., Dilon E., Pessin V., Pincas L. and Strauss A., 1951, Publ. Hlth. Rep., 66:327.
2. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,
APHA Inc., Washington, D.C.
3. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.
Q: Are you trading company or manufacturer ?
A: We are manufacture, mainly focusing on the development and production of microbial culture medium since 2000.
Q: How long is your delivery time?
A: Usually it takes about 7 working days after the deposit received, it depends on the quantity.
Q: Do you provide samples ? is it free or extra ?
A: Yes, we could offer samples of most products for free charge but do not pay the cost of freight.
Q: How to get quotation?
A: Please tell us the the products and the quantites you need, we will offer you our best prices.
Q: Can you design for us?
A: Of course, we accept various OEM and ODM orders.
Q: How do I pay?
A: You can pay us by wire transfer, WesternUnion, bank transfer, PayPal, etc.
Q: How many kinds of products do you have?
A: As many as 1000 kinds, which makes Hopebio to be one of the manufacturers which have the most complete microbial products series
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